The two musicians first clashed when 50 Cent included a puppet of Joe getting beaten up in his 'Piggy Bank' music video.
Cent reignited the feud in January when he told US TV show Rap City that he wanted to eliminate Joe from the "rap game".
However, Joe has insisted that he's not intimidated by the comments and claimed Cent was cowardly for not taking on the people who famously shot him nine times in 2000.
Joe told Complex: "He got shot by people who he knows and he doesn't do nothing about it.
"He gets criticised by people like Ja Rule, little Ja Rule, and he never even fought him one on one.
"He's going to come f**k with Fat Joe? Are you serious? He still don't leave his house. He's looking for attention, I'm gonna diss him. I'm gonna tell him to suck my d**k, everything you can think.
"I'm gonna tell him that. What does he want out of me? He's gonna tell me, 'Oh, Fat Joe, you're a sucka', and I'm gonna be like, 'Suck my d**k you punk a** motherf****r."