Bassist Eric Avery said he would take to the stage with singer Perry Farrell, guitarist Dave Navarro and drummer Stephen Perkins at the NME Awards in Los Angeles on April 23.
"I have always considered reunions to be a way to make a quick buck, and it sells short my own experience of it the first time around," Avery said in a statement. "The reason I started to even consider this is because it's honouring the past instead of trying to recreate it."
The band went through a turbulent break-up in 2004, with Navarro later saying the group "did not get along on any level".
Jane's Addiction will pick up the "Godlike Genius Award" from NME for their contribution to alternative rock later in the month.
The NME Awards have been held in the UK since the 1950s. This is the first time the music magazine has put on an event in the US.