Researchers found no conclusive evidence for its existence after reviewing 100 studies from the past 60 years, The Telegraph reports.
They claimed the concept of the G-spot, said to be a small area of the female body with a large number of nerve endings, is popular due to pornography and sex therapists.
"Objective measures have failed to provide strong and consistent evidence for the existence of an anatomical site that could be related to the famed G-spot," lead researcher Dr Amichai Kilchevsky from the Yale-New Haven Hospital in Connecticut said.
"Lots of women feel almost as though it is their fault they can't find it. The reality is that it is probably not something, historically or evolutionarily, that should even exist."
The findings, which were published in the Journal of Sexual Medicine, support a survey of 1,800 women by King's College London which concluded there was no evidence that the G-spot existed.
The G-spot was named after Ernst Grafenberg, a German gynaecologist who claimed to have discovered it back in 1950.