The sprinter - who won Sunday's (August 5) men's 100m final in an Olympic record time of 9.63 seconds - told journalists after his victory that the regulations "don't make any sense" to him.
Bolt, who said that he was prevented from bringing warm-up equipment including a skipping rope and stretching mat into the Olympic stadium at Stratford in East London, explained: "It has been different from Beijing. There are lots of rules, weird, silly rules that don't make any sense to me.
"I tried to wear my tie into [the Olympic Park]. They said, 'No'. I said, 'Why?' 'Because of the rules', they said. Then I wanted to bring my skipping rope in and they said 'no', because it's 'the rules'. These rules just don't make sense to me."
Bolt even claimed that he had been warned to abide by the track's rules as he warmed up moments before the 100m final, recalling: "I was about to run in the final and a guy was telling me to line up, to 'stay in a straight line'.
"I thought, 'Really? You're going to make me line up?' [They're] just silly rules."
Lord Sebastian Coe, who is chairman of LOCOG (London Organising Committee of the Olympic Games), has promised to investigate Bolt's claims.
"Skipping ropes are not expressly banned", Lord Coe confirmed. "I will look at this, I presume the skipping rope was a warm-up aid so I will look at that.
"I think some of it has been slightly lost in the translation. Every venue is different, there are different protocols."
Over 20 million people watched Bolt's 100m victory on the BBC, according to initial overnight data.
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