The dance troupe - who lost out to Diversity in the 2009 series of the show - joined Tesco staff and customers in an attempt that took place at 4.35pm yesterday (October 18).
Thousands of Tesco staff at 10 offices, depots and stores across the UK - including branches at London's ExCeL Centre, Leeds, Bangor, Dundee and Birmingham - simultaneously tried to stand on one leg unaided for two minutes in order to smash the previous record of 1,103 people, which was set in March by employees of ATEA (Denmark) in Copenhagen.
The attempt was also designed to help raise funds for Stand Up to Cancer, the new national fundraising campaign from Cancer Research UK, for which Tesco is a lead sponsor.
Independent witnesses oversaw the event and have submitted film, photographs and records of participation numbers to official adjudicators from Guinness World Records.
Members of the official body are currently analysing evidence from each of the venues to decide if a new world record has been created.
"Congratulations to all Tesco staff and customers who took part in this fantastic Guinness World Records record-breaking attempt," said Marlon Wallen, founder of Flawless.
"Keeping your balance for two minutes isn't easy but they did a brilliant job and helped raise vital funds for Stand Up to Cancer."
Claire Rowney, Cancer Research UK's Head of Corporate Partnerships, echoed Wallen's praise and added: "We are entering a 'golden age' of cancer research and every pound we raise takes us a step closer to achieving our goal to beat cancer.
"By boosting funding right now, the best research teams will be able to develop new treatments, bring cures faster and save more lives."
Channel 4 tonight aired a live Stand Up to Cancer fundraiser featuring comedy, entertainment and celebrity appearances. The campaign - hosted by Alan Carr, Davina McCall and Dr Christian Jessen - was organised by Gwenyth Paltrow in conjunction with Cancer Research UK, and has drawn support from big names including Kylie Minogue, George Clooney and Will Smith.
To find out more about the initiative, including how to donate to the cause, visit the official Stand Up to Cancer website.