The retailer removed the meal from its shelves on Tuesday (February 5) when it learned that Findus beef lasagne may have been contaminated.
The meat in the spaghetti bolognese meals was produced in the same Comigel factory in Metz, France as the Findus beef lasagne.
A Tesco Group spokesman stated: "The frozen Everyday Value Spaghetti Bolognese should contain only Irish beef from our approved suppliers. The source of the horsemeat is still under investigation by the relevant authorities.
"The level of contamination suggests that Comigel was not following the appropriate production process for our Tesco product and we will not take food from their facility again.
"We are very sorry that we have let customers down."
Last month, a sample from Tesco Everyday Value Beef Burgers was found to contain 21.9% horse DNA.
A number of other retailers, such as Lidl, Aldi and Iceland, have also been affected by the horsemeat controversy, as well as Burger King.