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The comic was performing at The Hexagon in Reading and, having finished his set, began flirting with the fans and posing for pictures.
According to The Sun, Brand then invited two female fans back to his dressing room and one of them left shortly afterwards.
25 minutes later the second girl reportedly came skipping out of the dressing room boasting "I just slept with Russell Brand!"
A source for the newspaper said: "The two girls had been shouting at Russell at the end of his live set and asking to go backstage. Both went and met him and he was saying some very raunchy things.
"One ran back out immediately - I'm not sure what shocked her. But the other one stayed in there."
