The band, which was created by Heroes actor Greg Grunberg, performed at Griffith Park, near the Autry National Centre for Netflix Live! On Location.
The Band From TV features Heroes star Adrian Pasdar and Desperate Housewives' James Denton on guitar, while House actor Jesse Spencer performed electric violin.
Guest vocalists included Hugh Laurie, Lost's Jorge Garcia, Hayden Panettiere, Teri Hatcher and Chuck star Zach Levi.
The event, which had an audience of 1,800, raised money for the Pediatric Epilepsy Project.
Speaking at the event, Grunberg said: "The epilepsy community has been hit so hard for so long with a stigma, I just want to associate anything cool, anything out of the box with it."