The Australian actor said film bosses were forced to rethink how to film the superhero's famous claws after he hurt Rebecca Romijn's stunt double.
Jackman, 40, said: "On the very first film we had real metal claws and the girl who was playing Mystique, Rebecca Romijn, her stunt double and I were doing a fight sequence in the Statue of Liberty.
"I went to stab her in the arm, which was part of the choreography, and she's meant to move her arm away and she forgot and the thing went right into her arm, like an inch.
"I was stunned. It was one of those things, it was so deep, there was a hole, there was no blood coming out and then like a minute later, it was like a geyser. I didn't faint and I thought she was going to freak out, but she just said, 'Yes! I have been stabbed by Wolverine!' Typical stuntwoman."
X-Men Origins: Wolverine, which hits cinemas on April 29, explores the early years of Jackman's character and his involvement in the government's Weapon X programme.