The former member of girlband Danity Kane was reported to have demanded car service for the shoot, and neglected to compensate the driver with a gratuity.
However, NOH8 co-founders Adam Bouska and Jeff Parhsley denied the allegations, telling Us Weekly: "We are happy and excited to have Aubrey O'Day [as part] of the NOH8 campaign.
"It is unfortunate that while doing something for the greater good someone found pleasure in trying to tear it down. Aubrey believes in marriage equality which is exactly what this campaign is about. We had a great time shooting her and we can't wait to release the PSA next week. Aubrey O'Day and the NOH8 campaign will continue to work together in the fight against hate and discrimination."
O'Day also replied to the rumours on her Twitter page, saying: "It's sad to think that people can't defend a just and honourable cause without being hated on! When has entertainment and profit become more important than the human heart!"
Aside from O'Day, the NOH8 advertising campaign is being supported by Ashlee Simpson-Wentz, Pete Wentz, Emmy Rossum and Lance Bass.