It had been reported that the Chelsea Lately host poked fun at Jolie and branded her a "homewrecker" during a show in New Jersey over the weekend.
However, in a message on her Twitter account, she said: "I have been making fun of Angelina Jolie for years. Period."
The comedienne also referenced the incident during a discussion on her E! show last night, saying: "They were talking about me on The View today and my Angelina Jolie tirade...
"Can I just say that I've been making fun of Angelina Jolie since she made out with her brother!"
She also said that the comment came from one of her comedy shows that she did "every weekend, all year for five years", prompting guest Josh Wolf to joke: "You should get some new material"
"It's true," she laughed. "If I have learned anything from this it is to write some new jokes!"
Chelsea Lately airs weeknights on E!