The event will feature 300 pieces of memorabilia and works of fine art from the actor's reservoir.
An Andy Warhol portrait of Mao Zedong, which Hopper shot two bullets through, is expected to fetch from $20,000 (£12,908) to $30,000 (£19,362) at the Christie's auction, reports Art Info.
Inspired by Vincent Price, Hopper starting collecting art in the 1960s. The Easy Rider star also befriended several artists during his life, including Warhol and Roy Lichtenstein.
Further artists represented in the sale include Wallace Berman, Bruce Conner, Julian Schnabel and George Herms.
The auction will feature posters from Hopper's films, including Speed, Blue Velvet and Apocalypse Now as well as an unbound script for Easy Rider.
The event will take place from January 11 to January 12 in New York City.
Hopper died last May after battling prostate cancer.