The broadcaster debuts the show on February 15 at The Y as part of the Leicester Comedy Festival.
The launch night is hosted by MC Jarred Christmas and features Isy Suttie, Josh Widdicombe, Carl Donnelly and headliner Brondan Burns. Tickets are available now and are priced from £10.
Absolute Radio chief operating officer Clive Dickens said: "Comedy is an integral part of our brand strategy. It has joined music and football as our third brand pillar.
"Live comedy is truly in the nation's hearts - who'd have thought that comedians would be selling out arena tours and the O2?"
Following its launch, Absolute Radio Comedy Live will become a monthly comedy night held at different venues around the country.