The Mean Girls star is seeking an injunction to stop the broadcasting of the rapper's hit song, as well as unspecified damages, TMZ reports.
The song lyric in question is as follows: "Hustlers move aside, so I'm tiptoein', to keep flowin' / I got it locked up like Lindsay Lohan."
Lohan claims that the line "causes [her] to be associated and identified in connection with defendants." Furthermore, the suit states that "the lyrics, by virtue of its wide appeal, condemnation, excoriation, disparaging or defamatory statements by the defendants about the plaintiff are destined to do irreparable harm to the plaintiff."
Finally, the actress says that her name is being "exploited for commercial purposes."
Last year, Lohan settled a similar lawsuit against E-Trade over alleged defamation of character for an unspecified sum of money.
The actress was reportedly offered the chance to front an anti-cheating website designed to name and shame unfaithful partners.
Watch the music video for 'Give Me Everything' by Pitbull, Ne-Yo and Afrojack below: