The Twilight actor holds on to the top spot, keeping his co-star Taylor Lautner in second position. Johnny Depp improves on his number six position in 2010, rounding off the top three.
David Beckham sits at number four, jumping an impressive 17 spots on last year, while Zac Efron is a new entry at five.
Also making the top ten are Gerard Butler, Alexander Skarsgard, Garrett Hedlund, Jared Followill and HRH Prince Harry.
New to the top 20 are actors Alex Pettyfer (13), James McAvoy (14), Henry Cavill (15), Ryan Reynolds (17), Colin Farrell (19) and Darren Criss (20).
Sherlock star Benedict Cumberbatch debuts at number 33, while Doctor Who's Matt Smith sits at 43.
Justin Bieber falls out of favour with voters, as he falls from number 7 to 45.
The full list appears in the November issue of Glamour magazine, out October 6.
Watch the trailer for The Twilight Saga: Breaking Dawn - Part 1 below: