Kardashian cited "irreconcilable differences" in court documents filed in Los Angeles, according to TMZ. The filings list the date of separation as today (October 31).
Kardashian's close friend Ryan Seacrest confirmed the couple's divorce on Twitter, writing: "Yes @kimkardashian is filing for divorce this morning. I touched base with her."
> Kim Kardashian on Kris Humphries divorce: 'I decided to end marriage'
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The couple tied the knot just 72 days ago in a $20m fairytale wedding in Montecito, California in front of other members of the Kardashian family, Seacrest, Lindsay Lohan and Eva Longoria.
Kardashian will reportedly be represented in the divorce proceedings by attorney Laura Wasser, who has previously aided stars such as Britney Spears, Angelina Jolie and Ryan Reynolds. She has asked that the couple split attorneys' fees, but will not be seeking spousal support from Humphries.
An insider has claimed that one major reason why the couple split is that they couldn't decide where to live following their wedding. Humphries reportedly wanted to move to his home state of Minnesota, while Kardashian hoped to settle down near her family in LA.
E!, who air Keeping Up with the Kardashians, said in a statement: "All of us at E! are surprised and saddened by this news, and we send our support to Kim and Kris at this difficult time.
"The Kardashians have always been uniquely open with all aspects of their lives from joyous events to intimate moments to heartbreaking transitions. E! will continue to share the fascinating real lives and larger-than-life existence of this close-knit family."
The reality star previously suggested during an interview with Jay Leno that both she and Humphries signed prenuptial agreements prior to their wedding.
Rumours of a possible split were fuelled last week, when Kardashian admitted that living in the public eye put a strain on her and Humphries's relationship.
Kardashian later said that she doesn't let media speculation on her love life upset her, and also insisted that she and Humphries always try to make time for one another.
Her first marriage to music producer Damon Thomas also ended in divorce in 2004.
> Kourtney, Khloe show support for sister Kim Kardashian after divorce