The former Two and a Half Men star insisted that Mueller, who was arrested last weekend on suspicion of felony cocaine possession, would never deal drugs.
"With the child support I give her... she's gonna deal drugs?" Sheen told TMZ. "Never gonna happen."
He went on to speak about his future with Mueller, with whom he has two children.
"We're interested in co-parenting... the kids need two parents," Sheen said.
A spokesperson for Mueller has said that all drug charges against her should be dropped.
Sheen bailed Mueller out of jail over the weekend, posting an $11,000 (£7,000) bond.
> Brooke Mueller 'to escape jail over cocaine arrest'