Takei, who played Mr Sulu in the original Star Trek, has released a video calling on Star Trek and Star Wars fans to unite in their love of sci-fi.
William Shatner had sparked controversy earlier this year when he claimed that Star Trek is the superior sci-fi franchise, with Carrie Fisher later responding that Shatner's comments were ridiculous.
In a newly-released clip called 'George Takei is the Broker of Star Peace', the actor warned fans of both franchises that their shared threat is the vampires of the Twilight films.
"Gone is any sense of heroism, camaraderie, or epic battle. In its place we have vampires that sparkle, moan and go to high school," Takei quipped.
The actor continued: "Now, I'm not above mixing in a little sex appeal to spice up the fantasy, but sci-fi fans be warned, there are no great stories, characters or profound life lessons to be found in Twilight... In Twilight, the only message that rings through loud and clear is, 'Does my boyfriend like me?'"
Takei closed the clip with a final plea to Star Trek and Star Wars fans, saying: "Let's us all just live and let live - long and prosperously. And, may the force be with you always!'
In September, Takei told Digital Spy that he would be willing to return to the role of Mr Sulu in a future Star Trek film.
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