In a poll compiled by PETA and news agency WENN, Jackson beat out director Cameron Crowe and reality star Kim Kardashian as the star most at odds with the organisation's aims.
"While the holidays may be known as the season of giving and peace on earth, that's not always the case for our animal friends," PETA spokeswoman Wendy Wegner told WENN.
"When Janet Jackson had her infamous wardrobe malfunction during Super Bowl XXXVII, at least what popped into view of 170 million onlookers belonged to her, unlike the animal skins she drapes herself in, which are as dead as her fashion taste and her career."
Speaking about fellow offenders Crowe and Kardashian, Wegner added: "Cameron Crowe went retro in the worst way for his latest film We Bought a Zoo, which relied on the performing-animal industry, in which the 'actors' are routinely neglected and even abused. He should have used modern computer-generated imagery. Exploiting live animals to tell a story about saving animals?
"And it took Kim Kardashian just 72 days of marital bliss to shed her husband Kris Humphries. It's too bad the socialite princess doesn't have the same penchant for shedding her animal skin collection. Unlike her sister Khloe, who starred in her own PETA anti-fur campaign, Kim just can't seem to divorce herself from fur and say 'I do' to a more compassionate wardrobe."
Jackson was previously slammed by PETA for modelling minks as BlackGlama's new 'What Becomes a Legend Most?' spokesperson.
The animal rights organisation also called for a warning on Crowe's We Bought a Zoo, describing the film as "dangerous and downright misleading".