The Food Network chef was first rumoured to be battling the disease in a series of tabloid reports in 2010, but has declined to comment on her health until now.
Deen has partnered with pharmaceutical company Novo Nordisk to launch the 'Diabetes in a New Light' programme, which develops strategies to help sufferers manage the illness.
"I was determined to share my positive approach and not let diabetes stand in the way of enjoying my life," she said in a statement promoting her new regiment.
Deen - who is famous for her high-calorie entrees and rich desserts – further explained that being diagnosed with diabetes won't force her to give up her favourite culinary delights.
She said: "I'm excited to team up with Novo Nordisk on this initiative to show others that managing diabetes does not have to stop you from enjoying the things you love."
Deen previously revealed that cooking helped her overcome agoraphobia when she was in her 20s.
The chef currently hosts the Food Network programmes Paula's Home Cooking and Paula's Best Dishes.
Deen will trace her family's history in an upcoming episode of the NBC series Who Do You Think You Are?.
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Watch a clip from Paula's Home Cooking below: