The 77-year-old comedian, who is best known for his cross-dressing role as Dame Edna Everage, began his acceptance speech by quoting Rupert Murdoch when he was in front of a government committee to face accusations of phone hacking last year.
According to The ABC, Humphries said: "This is the most humble day of my life." He then went on to joke about the phone hacking scandal, saying: "I had a friend very high up in the world of media who has been tapping the phones in Australia House for many years.
"He told me... I can't tell you his name, he is married to a Chinese girl. He said it was between me and Julian Assange and it turned out to be me."
After collecting the gong at Australia House in London, Humphries said: "Well, it's about time really, I think." He then joked that his alter ego Dame Edna would be very jealous of the award.
Humphries has previously been given the titles of Officer of the Order of Australia and Commander of the Order of the British Empire for his dedication to the theatrical industry.
UK wine writer Matthew Jukes won the 'Honorary Australian of the Year' prize, while fashion consultant Yasmin Sewell was named 'Young Australian of the Year'.
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Listen to Barry Humphries talk about Dame Edna Everage below: