The conservative organisation recently called for retail chain JCPenney to fire the talk show host as its spokesperson because of her sexuality, although JCPenney stood firm with its decision to hire DeGeneres.
DeGeneres has now spoken out about the controversy on a recent episode of her talk show.
She started off by saying how happy she was that Prop 8 was ruled unconstitutional in California and followed it with her response to One Million Moms.
"They wanted to get me fired, and I'm proud and happy to say that JCPenney stuck by their decision to make me their spokesperson," DeGeneres said, drawing applause and cheers from her audience.
"Which is great news for me because I also need some new crew socks," she joked.
DeGeneres then read One Million Moms' message on its Facebook page and comments from Facebook users who defended her.
DeGeneres ended the segment by thanking her supporters and stating the values she believes in.
She said: "Here are the values I stand for - I stand for honesty, equality, kindness, compassion, treating people the way you'd want to be treated and helping those in need. To me, those are traditional values. That's what I stand for."
Watch the full video of Ellen DeGeneres's response to One Million Moms below: