The contest saw them battle over who could cook a basic three-egg omelette in the shortest time.
Chef James Martin, the programme's presenter, said: "The challenge strikes fear in chefs like you've never seen before - you get three Michelin-starred chefs that often come on the show and haven't cooked an omelette for 20 years.
"There's sheer horror on their faces when they've got to do it in front of millions of people - and I think the footballers were the same as well: a little bit shocked when they saw they had to actually do it! I think they expected the eggs to come out of a packet! They were all very good sports."
The programme's makers have promised some good-natured "foul play" from the footballers and "one or two players head[ing] for a red card".
In taking on this challenge for Sport Relief, the England players join fellow sportsmen Freddie Flintoff, who broke 14 world records in 12 hours, and rugby stars including Sam Tomkins and Jon Wilkin who stripped off for the charity event.
With other famous faces from Prince Harry to John Bishop and Olly Murs to The Saturdays getting involved in the upcoming charity weekend, members of the public are being encouraged to join in with their local Sport Relief Mile on Sunday, March 25.
To find out more about Sport Relief and running your local mile, visit http://www.sportrelief.com.
The footballers' Saturday Kitchen Omelette Challenge will be shown on the Sport Relief night of TV on BBC One and BBC One HD on Friday, March 23.
Look through a gallery of images from the programme below: