The temporary restraining order was filed against Linda Ransom on Friday (May 25) in a Los Angeles County court.
In the court filing, the CSI star alleged that Ransom has been stalking him since 2001.
He claims the woman has shown up at several of his theatre performances in both LA and NY, and has followed him in public and shown up at his home in LA uninvited.
In court documents obtained by TMZ, the actor says: "I am frightened of Ms Ransom and feel she poses a serious threat to my personal safety."
Goldblum's previous restraining order against Ransom expired while she was being treated at a mental institution. He was denied a renewal of the order in 2010 after she had just been released.
A judge ruled that Ransom would have to make a "fresh threat" before any legal actions could be taken.
Goldblum recently joined the cast of the Sarah Silverman pilot Susan 313