The Mr Bean star is set to play one of the seven teachers working in an English language school for foreigners in the 1960s.
The new West End production will begin performances at the Vaudeville Theatre on January 23, 2012 before an official opening on January 29.
Prior to this, the production will visit Brighton's Theatre Royal on January 8-12 and Theatre Royal Bath on January 14-19.
Last year, Atkinson was involved in a crash when his McClaren F1 sports car hit a tree in Cambridgeshire, England. He was hospitalised for a shoulder injury.
Atkinson called the incident a "misjudgement" but plans to drive the McClaren F1 again.
"I'll certainly be driving the car again. It hasn't put me off the car but you've got to learn your and your vehicle's limitations, so I think it's definitely a lesson learnt and I would hope to not be in the same situation [again]," he explained.
Watch Rowan Atkinson in the trailer for Johnny English Reborn below: