New York City's W.i.P. was temporarily shut down for code violations following the injuries of several club-goers in the June 13 altercation.
The State Liquor Authority (SLA) has now filed 13 charges against W.i.P. over the alleged Brown and Drake brawl as well as other incidents.
W.i.P. security are accused of not acting quickly enough to break up the rappers' fight, and separate drug and noise violations.
SLA rep William Crowley told The New York Post: "They're absolutely in jeopardy of losing their licences. They have a number of prior charges, too, so that comes into play when we're looking at taking action."
W.i.P. owners have been ordered to answer the charges by July 11, but have yet offered no comment.
NBA star Tony Parker has already filed a $20m (£12.8m) lawsuit against W.i.P. for suffering a scratched cornea in the brawl.
Another bystander who was injured in the clash has also threatened to sue over her own injuries.