The comedian was mocking England goalkeeper Joe Hart when he managed to hurt his thigh, reports The Sun.
McIntyre was poking fun at Hart's attempt to save Italy's Andrea Pirlo's chipped penalty last Sunday in front of his Cork audience.
However, he later returned to the stage on crutches and had to finish the rest of the show sitting down.
He joked to the audience: "You're entitled to a refund. It isn't the show you paid for — unless you enjoy casualty waiting rooms."
Michael McIntyre recently mocked BBC One's The Voice UK, saying that "people aren't interested" in the show.
Watch Michael McIntyre talk about football below: