The 51-year-old's marriage to the older sibling of his Devil Wears Prada co-star was confirmed by his representative, E! News reports.
The announcement comes after Tucci was spotted wearing a wedding band at the New York premiere of new movie Hope Springs on Monday (August 6).
The Oscar nominee was introduced to his literary agent wife by Emily and proposed to her in November 2011.
Tucci lost his first wife Kate to breast cancer in April 2009. The couple had three children together, twins Isobel and Nicolo and youngest daughter Camilla, while Tucci also helped raise her two children from a previous marriage.
Emily, meanwhile, has been married to The Office actor John Krasinski since July 2010.