Also added to the lineup for the We Are Most Amused night on Wednesday, November 28, supported by Novae, are E4 comedy trio The Midnight Beast.
© David Davies/PA Wire/Press Association Images
© Comic Relief
Other comedy talent collaborating at the event include trust supporters Stephen K Amos, Sanjeev Bhaskar, Omid Djalili, Milton Jones, Patrick Kielty, Helen Lederer, Justin Lee Collins and Dame Edna Everage, while Ben Elton will be writing a sketch.
"I'm looking forward to this event hugely - a fantastic lineup for a very important cause," said Hart.
"The Prince's Trust is vital at a time when so many young people are disillusioned and unemployed.
"Raising money and sharing a stage with Rowan Atkinson - it's going to be a great night."
Collins added: "This is going to be a great event - and even better because it is for The Prince's Trust, a cause close to my heart.
"I was so lucky to get my break in life, but there are so many young people who fear they'll never get theirs.
"That's why I support The Prince's Trust. I want young people to know that they can pursue their passions, in the same way that I have."
Tickets are priced from £20 to £125 and are available now.
