The British publication announced on Monday (October 8) that the royal tops its list of male celebrities for 2012.
Prince Harry's reputation for occasionally scandalous behaviour was referenced on Tatler's cover, with the magazine referring to him as "Dirty Harry".
The Prince most recently made headlines when photographs from a naked romp in Las Vegas in August were leaked to the press.
Buckingham Palace confirmed last month that it will not pursue an official complaint over The Sun's decision to publish the images.
Prince Harry is currently on a four-month deployment in Afghanistan with the 100-strong 662 Squadron, 3 Regiment, Army Air Corps.
Tatler is a UK publication with an illustrious history dating back to 1709. The most recent incarnation of the magazine was founded in 1901.