The British boyband - Harry Styles, Niall Horan, Zayn Malik, Louis Tomlinson and Liam Payne - signed a multi-million dollar deal with the drinks company back in September.
Watch One Direction's Pepsi commercial below:
The advert premiered during last night's (October 10) edition of The X Factor USA and features One Direction meeting NFL star Drew Brees backstage at a talk show.
The band and the American football player face off over a can of Pepsi, before One Direction agree to let Brees in the band if he hands over the drink.
A burst of One Direction's latest single 'Live While We're Young' also plays during the advert.
Recent reports claimed that the band - who were put together on The X Factor in the UK - have made nearly £100m ($160m) in two years.
New! Digital Spy breakfast show - watch Alex Fletcher discuss the day's biggest stories including the Rooneys' baby joy, Cheryl Cole's long-awaited autobiography, what's next on Doctor Who and more:
