The singer addressed a pledge made by Romney in the first presidential debate to cut funding to PBS if he is elected.
"I'm going to stop the subsidy to PBS," the former governor told debate moderator Jim Lehrer, a PBS host. "I'm going to stop other things. I like PBS, I love Big Bird. Actually, I like you too. But I'm not going to... keep on spending money on things to borrow money from China to pay for."
Streisand joked about Romney's presidential bid pledge during her three-hour set at the Barclays Centre on Thursday (October 11).
"I love Big Bird and I hope no one tells Romney how to get to Sesame Street or Pennsylvania Avenue," Streisand said, adding of Romney: "He's a good actor. He's a good actor. A chameleon."
[Presidential candidate Mitt Romney (Republican) and current US president Barack Obama (Democrat) shake hands at their first televised debate]
Streisand - a long-time Democratic donor - went on to discuss her feelings about the US presidential election, The AP reports.
"I'm not going to tell you who to vote for, but... if you believe in affordable health care and women's rights... if you want to move forward and not back, you know who to vote for," Streisand said in an apparent statement of support for Barack Obama.
Romney picked up a high profile endorsement this week, when Mean Girls star Lindsay Lohan revealed that she will vote for the Republican ticket.
"I just think employment is really important right now… So as of now, [I support] Mitt Romney. As of now," Lohan commented.
The US presidential election will be held on Tuesday, November 6.