The pop singer told Now magazine that he is sick of fans speculating on his and bandmate Harry Styles's sexuality.
Tomlinson said: "A lot of them are so wrapped up in the conspiracy. Let me tell you now, they'll find a way to put some twist on this interview."
The 20-year-old has gotten into heated battles via Twitter with fans who claim that his relationship with girlfriend Eleanor Calder is merely a stunt to hide a romance with Styles.
"I think it's pretty obvious when you see me and Eleanor together that it's real. Think of the amount of time I spend with her. It's crazy that I even have to say it's genuine.
"The truth is, these people aren't our real fans.That's the way I like to look at it.'
Harry Styles, who has been linked to several stars and previously dated Xtra Factor host Caroline Flack, recently revealed that he once thought he got a girl pregnant.
Tomlinson's full interview is available in the latest edition of Now magazine.
Watch the video for One Direction's new single 'Little Things' below: