The Wolf of Wall Street actor welcomed family and friends to New York City's The Darby, where a percentage of every bottle of wine sold raised money for the less fortunate.
Beyoncé and husband Jay-Z, Mark Ruffalo, Miranda Kerr, Robert De Niro and DiCaprio's Django Unchained co-star Jamie Foxx were in attendance, according to Page Six.
The celebrity guest list for DiCaprio's birthday bash also included Chris Rock, Edward Norton, Emma Watson, Martin Scorsese and Russell Simmons.
All proceeds from the fundraiser went to charities supported by The Leonardo DiCaprio Foundation, with the American Red Cross receiving a $500,000 (£314,782) cheque.
DiCaprio turned 37 on Sunday, November 11.
He will next appear on the big screen with Foxx in Django Unchained, opening on Christmas Day in the US and on January 18 in the UK.
Watch a trailer for the film below: