The event, which raises money to help disadvantaged children, has already beaten last year's total of £26.3 million, and is expected to exceed the current total of £26,757,446 once all donations are collected.
Last night's telethon was hosted by Sir Terry Wogan, alongside Fearne Cotton, Tess Daly and Nick Grimshaw.
Highlights included appearances by Girls Aloud and One Direction, as well as a spoof performance of Psy's 'Gangnam Style' by Zara Phillips and teammates from the Olympic 3 Day Eventing team. BBC Newsreaders took part in a Top Gear challenge and Lord Alan Sugar starred in a special EastEnders.
There was also a Doctor Who minisode and the debut of the trailer for the show's Christmas special 'The Snowmen'.
This year's event took place following months of sexual allegations against late BBC star Jimmy Savile, which has engulfed the corporation in a number of scandals.
Wogan made reference to the controversy during last night's programme, saying: "We know that children are vulnerable. In the news in recent weeks there has been an awful reminder of just how true that can be.
"We have been supporting... victims all of all kinds of abuse, including sexual abuse, for many years now. We will continue to do so."
Girls Aloud have reunited to record this year's Children in Need single, 'Something New/Beautiful Because You Love Me'.