The legendary singer donated $100,000 (£61,500) to Jasmine Faulk during a live show at the Nokia Theatre in Los Angeles on Saturday (December 15).
Wonder invited the 10-year-old and her father on stage before presenting her with a giant cheque, reports TMZ.
He then proceeded to perform his classic hit 'Isn't She Lovely' in tribute to her and her fight against the disease.
During the performance, Faulk grabbed Wonder's microphone, proclaiming: "I love you, Stevie!"
Charlie Sheen previously donated $75,000 to the girl's fund last week after he found out about her story from stunt double Eddie Braun, who had also given $25,000.
Jasmine Faulk's Hermosa Beach police officer father has also been working for Stevie Wonder since 1997.