Directed by Danny Boyle, the event was chosen by 55.4% of people in the survey. The Summer Games' Super Saturday - which saw gold medals for Jessica Ennis, Mo Farah and Greg Rutherford - came second with 11.3%
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Away from the Olympics, Christopher Nolan's final chapter in his Batman trilogy - The Dark Knight Rises - came third with 10.9%.
Another major movie event, James Bond's 50th anniversary comeback with Skyfall, was fourth with 7.7%.
The Queen's Diamond Jubilee rounded out the top five with 6.5% of the vote.
Entertainment Highlight of the Year
1. London 2012 Opening Ceremony - 55.4%
2. Super Saturday: Gold for Farah, Ennis & Rutherford - 11.3%
3. The Dark Knight Rises ends the Batman Trilogy - 10.9%
4. James Bond's anniversary triumph with Skyfall - 7.7%
5. The Queen's Diamond Jubilee - 6.5%
More than 25,000 votes were registered in Digital Spy's end of year reader poll, held to decide the ultimate in entertainment and tech from the last 12 months.
Photo gallery - London 2012 Olympics Opening Ceremony:
Copyright: PA Images Anthony Devlin/PA Wire
