The Aerosmith singer and Brady announced their engagement one year ago, but reports of problems between the pair have been prevalent for several months.
Tyler apparently attempted to reconcile over the holidays, but an informant has told Page Six that the couple were just not able to mend fences.
"It's over for good this time," the insider said. "Steven was just not that nice to her in the end. His family and entourage never really accepted her and she can do better."
The former American Idol judge has not yet commented on reports of another split.
Tyler was previously married to Cyrinda Foxe from 1978 to 1987 and Teresa Barrick from 1988 to 2006.
He openly discussed his relationship with Brady over the last year, including joking that they experimented with bondage.
Watch Aerosmith perform 'Crazy' below: