Host James Corden joked about the One Direction star's relationship with Caroline Flack by asking him which older lady in the audience he had his eye on tonight.
Styles famously dated Flack - 14 years his senior - for several months before they split last year. She most recently attended his 19th birthday party.
"Sharon Osbourne is here, Annie Lennox is here... who have you got your eye on?" Corden teased, causing an embarrassed Styles to hide his head.
Later on in the evening, Osbourne herself took a shot at the singer while presenting the 'International Female' award to Lana Del Rey alongside Dermot O'Leary.
"Where is that little Harry Potter boy?" she asked. "Has he got his little magic stick with him tonight?"
She then clarified: "That's his willy."
Styles took to the stage at the ceremony to perform 'One Way Or Another (Teenage Kicks)' with his One Direction bandmates.
Photo gallery - Brit Awards 2013 in pictures:
Copyright: PA Images Yui Mok/PA WireWatch Little Mix, Labrinth and Ed Sheeran chat to Digital Spy at the Brit Awards below: