The boyband members were overcome with emotion as they stepped foot inside a hospital emergency ward for a video to be shown on tomorrow night's Let's Dance for Comic Relief.
In the Digital Spy exclusive pictures, Styles buries his head in his shirt and a red-eyed Payne wipes away tears as he comforts a sick child at their bedside.
The pair make a heartfelt plea in the clip for viewers to donate £5 to Red Nose Day, which could pay for a mosquito net to protect mothers and their children.
Over half a million people die every year from malaria in Africa, with 85% of the victims being children.
Speaking about One Direction's life-changing trip to visit those helped by Comic Relief, Payne said: "Going to Ghana to see for ourselves the difference money raised through Red Nose Day can make was a real eye opener and an emotional experience for all of us."
Back in the UK, Styles continued to help this year's Comic Relief appeal by creating an array of Red Nose Day cakes to sell at One Direction's rehearsals earlier this week.
The group have also donated the clothes they wore in Africa to Comic Relief to sell on eBay as part of the band's #1D4RND campaign, and recorded this year's official Comic Relief single, 'One Way Or Another (Teenage Kicks)'.
One Direction released the video for the track on Thursday (February 21) and later explained to DS that they chose to film it on tour because they didn't feel right "spending loads of money" after their Africa trip.
One Direction's official Red Nose Day single 'One Way or Another (Teenage Kicks)' has already gone to number one in the charts in 63 countries and is available to download from iTunes now, with the physical CD available to buy from Sainsbury's stores nationwide.
The second of One Direction's moving films made during their Red Nose Day visit to Africa will be shown on Let's Dance for Comic Relief on Saturday (February 23) on BBC One at 7pm.
Watch the video for One Direction's 'One Way Or Another (Teenage Kicks)' below: