Dr Martin Braun said the actress - who has claimed her look is "completely natural" - was harming the industry by her "enthusiastic" use of the anti-wrinkle treatment.
He told a cosmetic medicine conference in Australia: "Nicole seems to get her Botox done two or three weeks before a big event so when she, for instance, goes up on stage to collect her Academy Award she looks frozen and strange.
"She looks like a bat with too much of a brow lift, the middle of the brow's been dropped. She's crying when she accepts her Oscar but nothing is moving.
"That's really doing nothing to help our job because we've got women coming to us saying that's what we don't want to look like."
Dr Gabrielle Caswell from the Cosmetic Physicians Society of Australasia said industry insiders believe celebrities such as Kylie Minogue and Angelina Jolie also use Botox, with Jolie described as "a definite".
Dr Braun claimed that 99% of Hollywood stars have the treatment, adding: "They just don't like admitting it, that's all."