Speaking to Access Hollywood, the spokesperson confirmed that the Wrestler star would be making an appearance at the pay-per-view event in Houston, Texas on April 5.
The Oscar-nominated actor had previously expressed his interest in attending, telling Access: "I want to [do it]...I'm talking with [former WWE star] Rowdy Roddy Piper about it."
However, a representative for the 56-year-old later downplayed his involvement, insisting that work commitments would prevent him from participating.
During a taping of this week's Monday Night RAW, WWE star Chris Jericho fuelled speculation that the actor had reconsidered his position, after issuing a challenge to wrestling legends including Piper.
He said: "You say you're going to be at Mania, I'm going to buy you a front row seat, because I want you to see up close and personal what's going to happen to all your friends.
"And unlike your movie The Wrestler, the ending of this isn't going to be ambiguous. It's going to be tragic."
Representatives for Rourke have yet to comment.