The reality star said she would never do anything to jeopardize her friendship with Ronnie after being quizzed about her co-star on Late Night with Jimmy Fallon.
When asked if there were any sparks between them, she responded: "Ugh, what? A lot of people assume that. He's just a great friend."
Fallon then pointed out the scene when Ronnie broke up with girlfriend Sammi Giancola and JWoww comforted him by saying he was better alone.
"Me and Ronnie just knew each other, we grew up in the same area," replied JWoww. "He's such a great friend. We would never jeopardize... We'd never look at each other like that. He's someone I can confide in."
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As for Ronnie's current status with on-off girlfriend Sammi, she replied: "I personally don't know. I knew they were doing good and they you wake up and see them in different corners and you have to wonder."
Jersey Shore kicked off its fourth season, which sees the cast relocating to Florence, Italy for the summer, to record viewing figures earlier this month.
Last week's episode saw Sammi admitting that she still had feelings for Ronnie, and although he felt the same way, he chose not to act on his feelings after remembering how difficult their break-up in Jersey had been.
Jersey Shore airs Thursdays at 10pm on MTV.
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Watch more of JWoww talking to Jimmy Fallon below: