Cast and crew had their first practice runs yesterday (October 2) ahead of the hour-long 40th anniversary special, which will air on ITV1 later this month.
Lucy Pargeter, who plays Chas Dingle, tweeted last night: "#EmmerdaleLive 1st day of rehearsals done!!!!! WOW, OUCH and hope your gonna love it??? And relax…"
Meanwhile, Sian Reese-Williams - better known as Gennie Walker - confessed: "I think it's hit me today. YIKES."
The Emmerdale team will have 11 days of rehearsals for the live episode in total, working for 10 hours across each of the days.
61 cast members are involved in the challenging broadcast, while 350 supporting artistes will also be required.
Additionally, 70 crew members will be working - almost double the number for a regular Emmerdale shooting day.
Emmerdale Live director Tony Prescott commented: "The problem we have with trying to create a rehearsal schedule for something like this is that Emmerdale is a serial drama. If we take that time out of the schedule, when do the other episodes get made?
"It would be nice to have a lot more time to rehearse, but [series producer] Stuart Blackburn has distinctly said, 'There is no point in doing a live and getting an audience bounce of millions if we don't produce the same drama the following night'.
"Producing the drama that will play out following the live is just as important as the live."
Emmerdale's 40th anniversary week kicks off on Monday, October 15 and show bosses have confirmed that a popular character will be killed off in the live episode.