Emmerdale will go live on Wednesday, October 17 from 7pm, airing in an hour-long timeslot on ITV1.
> 'Emmerdale' 40th anniversary - full coverage
Show bosses have promised that the special edition will explore "every aspect of life" as it features two wedding celebrations, the cries of two newborn babies bringing joy to their loved ones - and the shock death of one villager in a tragic storyline twist.
The Emmerdale team have been busy preparing for the live episode over the past few months, but official rehearsals for cast and crew began yesterday (October 2).
In total, there will be 110 hours of rehearsals for the show's stars, spanning over 11 days.
61 cast members are involved in the challenging broadcast, while 350 supporting artistes will also be required.
Emmerdale kicks off its 40th birthday week on Monday, October 15.