Tom Lister made his final appearance as the calculating businessman, who was twice hit on the head by a brick, first by Chas Dingle and then by Cameron Murray.
> Emmerdale Tom Lister "thrilled" with Carl King exit plot
> Emmerdale 40th anniversary live episode - as it happened
Carl was initially wounded following a fight with his ex Chas (Lucy Pargeter), which he started upon learning that she lied about wanting to run away with him.
Then Cameron (Dominic Power), who'd had a confrontation with Carl in the church earlier in the evening, returned to finish off the job.
Cameron found Carl and tried to force him to promise not to send raunchy photos of him and Chas to Debbie.
> Emmerdale Nick Miles: 'No mistakes in live episode'
> Emmerdale: Backstage at the live episode - stream
Shrugged off by an arrogant Carl, who refused to agree to Cameron's demands, Cameron hit him from behind as he parted with the famous last words: "I'm indestructible!"
Bosses have managed to keep the identity of tonight's victim hidden since announcing the death in September.
Carl, whose funeral will take place next Friday, was the first King to arrive in Emmerdale in 2004, with Lister having clocked up eight years on the ITV soap.
Digital Spy readers rightly predicted that Carl would die in the broadcast, with nearly 50% of fans believing that Lister's alter ego would perish instead of the likes of Cain Dingle (Jeff Hordley) and Alan Turner (Richard Thorp).
Emmerdale Live also featured the live births of Gennie and Nikhil's baby girl as well as Debbie and Andy's baby boy.
The hour-long episode also focused on the aftermath of Declan and Katie's wedding, which culminated with a scene showing Declan's sister Megan pushing bride Katie's face into the cake.
Emmerdale's 40th anniversary celebration continues tomorrow in the show's usual Thursday double bill, starting from 7pm and concluding at 8pm.
> Read more about Emmerdale's 40th anniversary here
> Emmerdale Rik Makarem reveals live episode improvisation
> Emmerdale live episode thrills 8.8 million