The actors - better known as John Barton, Nikhil Sharma, Carl King and Andy Sugden on the soap - took on the gruelling challenge in a bid to raise £100,000 for Macmillan Cancer Support.
Joined by Emmerdale's executive producer Steve November and a group of professional cyclists, the foursome set off from the ITV1 show's village set on Friday evening with the aim of reaching EastEnders within 24 hours.
However, as we reported on Saturday, the stars smashed their target by completing the challenge in little over 20 hours.
Upon arrival at EastEnders, the Emmerdale team were greeted by Walford cast members Adam Woodyatt (Ian Beale), John Partridge (Christian Clarke), Marc Elliott (Syed Masood), Shona McGarty (Whitney Dean) and Matt Lapinskas (Anthony Moon).
Woodyatt commented: "Congratulations to the Emmerdale team, as it is a fantastic achievement. To do this bike ride in less than 24 hours is amazing and it's for a great cause. It's just a shame The Vic's not open as they could do with a pint!"
The Emmerdale to EastEnders challenge was carried out in memory of the rural drama's late series producer Gavin Blyth, who passed away last year following a short battle with cancer.
James Thornton said: "We are all overjoyed that we have managed to complete this massive cycle challenge in less than 24 hours to raise £100,000 for Macmillan Cancer Support. Gavin left a giant hole at Emmerdale so we did this to remember him and to try and raise money for Macmillan.
"It was exhausting, but with our fantastic supporters cheering us along the way and knowing that every penny we raised will directly help people affected by cancer, it was worth every mile."
Donations for the challenge can still be made via a dedicated JustGiving page. Alternatively, a £5 donation can be made by texting 'RIDE66 £5' to 70070.
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