Yesterday's instalment of the teen soap saw schizophrenic student Newt (Nico Mirallegro) attempting to take his own life after making a suicide pact with new friend Rae (Alice Barlow). The troubled character jumped from an abandoned warehouse into cold dockland water while on the run.
Earlier this week, 15-year-old Neve Lafferty and 14-year-old Georgia Rowe died after jumping into the River Clyde from the Erskine Bridge.
A spokesman for Bishopton's Good Shepherd Care Home, where the teenagers lived, has now told The Sun: "The decision to air this show is likely to cause further distress."
The network confirmed that it would transmit the episode as planned hours before broadcast yesterday.
Speaking at the time, a Channel 4 representative said: "Any similarities are entirely coincidental and we have carefully considered how best to proceed. We feel it is appropriate to continue with the transmission of these episodes as this is not a one-off programme but an established and long-running series.
"The audience will be familiar with the character of Newt and this plotline, which has been both trailed and promoted, has been developing over a number of weeks.
"Hollyoaks has a strong track record of dealing with sensitive issues, and the transmission will be followed by a programme support announcement directing viewers to a 24-hour helpline."
The aftermath of Newt's shock plot twist will unfold on screen next week.