Walton, who plays Loretta Jones on the Chester-based serial, shared the news with fans on her Twitter page this morning and confessed that she was feeling emotional over her exit.
The actress wrote: "I'm sharing my last day with Kevin Sacre and Gerard McCarthy!! Aw sad times :-( xxx."
In a later post, she added: "Am now blubbing wreck! Gonna miss everyone here so much!"
The departures of Walton and her screen love interest Sacre (Jake Dean) were announced in March following the arrival of Hollyoaks' new series producer Paul Marquess. McCarthy, meanwhile, is bowing out as Kris Fisher in order to pursue new projects.
Writing on his own Twitter page today, McCarthy admitted: "Am close to tears already! Sad I know... prepared myself yesterday by getting the Glee box set for tonight!"
A number of new faces will arrive on Hollyoaks in the coming months to fill the gaps left behind by departing cast members.