Show bosses have confirmed that Alex Carter is to bow out from the teen drama after nearly a year back on screen as Lee Hunter, while Dean Aspen and Ashley Margolis have also chosen to depart their roles as double act Duncan Button and Ricky Campbell.
After spending a decade in soapland while starring in Hollyoaks and Emmerdale, 28-year-old Carter is now hoping to pursue other opportunities.
Speaking of his decision, the actor told DS: "After ten years in soap, it feels like the right time to find out what else is out there."
He laughed: "I'm so grateful to Hollyoaks for giving me my first break and I'll be gutted to leave behind some really good friends that I've made in the cast and crew, but I'm sure I'll keep in touch with the ones I like!"
"I have had an absolutely brilliant time playing Duncan - I feel like Hollyoaks have paid me to laugh," Aspen said. "But, after two years, I really feel the time is right for a new challenge. I will be very sad to leave but hopefully the door will be left open and you just don't know what could happen in the future. I'd love to think Duncan could return."
Margolis added: "I'm not sure if I'm crazy or brave but yes, I think it's time for me as an actor to move on. I could very happily stay forever in my comfort zone - Hollyoaks is a great place to work. But with Dean moving on it just feels like the right time. Ricky has been a great character and I've learnt a lot in two and a half years. Landing a role on Hollyoaks at 15 was a dream in itself but I've always wanted to have a broad acting career so it's now or never."
Details of storylines for the trio's respective departures have yet to be revealed.
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